Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

ABSTRACT: Multiple Myeloma (MM) patients suffer disease relapse due to the development of therapeutic resistance. Increasing evidence suggests that immunotherapeutic strategies can provide durable responses. Here we evaluate the possibility of adoptive cell transfer (ACT) by generating ex vivo T cells from peripheral blood mononuclear cells (PBMCs) isolated from MM patients by employing our previously devised protocols. We designed peptides from antigens (Ags) including cancer testis antigens (CTAs) that are over expressed in MM. We exposed PBMCs from different healthy donors (HDs) to single peptides. We observed reproducible Ag-specific cluster of differentiation 4⁺ (CD4⁺) and CD8⁺ T cell responses on exposure of PBMCs to different single peptide sequences. T

SUBMITTER: Vardam-Kaur T 

PROVIDER: S-EPMC8487724 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature