Proteomic profiling of extracellular vesicles and particles reveals the cellular response to cisplatin in NSCLC.

ABSTRACT:

Background

Cisplatin-based chemotherapy is a therapeutic strategy against non-small cell lung cancer (NSCLC). However, cancers relapse after chemotherapy due to a dormant state of residual cancer cells. Extracellular vesicles and particles (EVPs) are active carriers of proteins and nucleic acid. Here, we aimed to study the molecular alterations and proteomic characteristics of EPV in dormant and reactivated cancer cells induced by cisplatin.

Methods

We used a short-term single dose of cisplatin to induce the dormant and reactivated cell status. We examined the gene expressional profiling and proteomic profiling of EVPs from dormant and reactivated cancer cells by RNA-sequencing and LC-MS/MS.

Results

We found substantial changes in gene expression and protein level in

SUBMITTER: Xu J 

PROVIDER: S-EPMC8487815 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature