A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae.

ABSTRACT: Klebsiella pneumoniae carbapenemase genes (bla _KPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for bla _KPC genes and investigations into the molecular characteristics of bla _KPC positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for bla _KPC was established. This protocol could be completed at 39°C in 15-20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The bla _KPC RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 b

SUBMITTER: Zhang W 

PROVIDER: S-EPMC8488121 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature