Dataset Information

A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae.

ABSTRACT: Klebsiella pneumoniae carbapenemase genes (bla _KPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for bla _KPC genes and investigations into the molecular characteristics of bla _KPC positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for bla _KPC was established. This protocol could be completed at 39°C in 15-20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The bla _KPC RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 bla _KPC positive isolates were identified from each sample, of which 23 isolates were K. pneumoniae. ST11 with bla _KPC-2 was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of bla _KPC positive carbapenem-resistant K.pneumoniae poses significant challenges for public health control.

SUBMITTER: Zhang W

PROVIDER: S-EPMC8488121 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Publications

A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae.

Zhang Weiwei W Feng Yanling Y Zhao Hanqing H Yan Chao C Feng Junxia J Gan Lin L Cui Jinghua J Liu Shiyu S Zhang Rui R Du Shuheng S Li Nannan N Xu Wenjian W Han Juqiang J Li Rongkuan R Xue Guanhua G Yuan Jing J

Frontiers in cellular and infection microbiology 20210920

Klebsiella pneumoniae carbapenemase genes (blaKPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for blaKPC genes and investigations into the molecular characteristics of blaKPC positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for blaKPC was established. This protocol could be ...[more]

PMID: 34616692

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Project description:Hypervirulent Klebsiella pneumoniae (hvKp) is a major human pathogen associated with liver abscess, pneumonia, meningitis, and endophthalmitis. It is challenging to differentiate hvKp from classical Klebsiella pneumoniae (cKp) using conventional methods, necessitating the development of a rapid, sensitive, and convenient assay for hvKp detection. In this study, we constructed a recombinase-aided amplification (RAA) method targeting hvKp genes peg344 and rmpA, and also analyzed the pathogenic characteristics of hvKp. We optimized the reaction temperature and system, and evaluated its sensitivity, specificity, and clinical application. The primer and probe sets peg344-set1 and rmpA-set2 delivered significant fluorescent signals at 39°C with the shortest gene amplification times (sensitivity: 20 copies/reaction). This RAA assay showed no cross-reactivity with 15 other common pathogenic bacteria. Its applicability was confirmed by the evaluation of 208 clinical specimens, of which 45 were confirmed to be hvKp. The sensitivity and specificity of the RAA assay were both 100% compared with real-time PCR as the reference standard. To verify the assay, we also assessed the diversity of molecular characteristics among the hvKp isolates and identified serotype K1 and sequence type ST23 as the dominant clone. Virulence factors iroN and iutA were highly associated with virulence level. In conclusion, our novel RAA assay is a powerful tool for early diagnosis and epidemiological surveillance of hvKp. IMPORTANCEKlebsiella pneumoniae is the most common opportunistic bacterial species and a major threat to public health. Since the 1990s, hvKp has received increasing attention from public health officials and infectious disease specialists. Hypervirulent strains differ from classical strains in terms of phenotypic features and clinical outcomes. It is hard to identify hvKp from cKp using the conventional methods including colony morphology analysis, serum killing assays, mouse lethality assays, string tests, and real-time PCR. In this study, we established a rapid, sensitive and convenient recombinase-aided amplification assay for hvKp detection targeting virulence genes peg344 and rmpA. Our RAA assay provides an important tool for the rapid diagnosis of infectious diseases caused by hvKp, particularly in primary laboratories.

Dataset Information

A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae.

Publications

A Recombinase Aided Amplification Assay for Rapid Detection of the <i>Klebsiella pneumoniae</i> Carbapenemase Gene and Its Characteristics in <i>Klebsiella pneumoniae</i>.

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