METTL3 Is Involved in the Development of Graves' Disease by Inducing SOCS mRNA m6A Modification.

ABSTRACT:

Objective

Epigenetic modifications in RNA are known to play critical roles in cell differentiation through regulating expressions of some key genes including members of the suppressor of cytokine signaling (SOCS) family. The present study aimed to unveil the relationship of SOCS mRNA methylation induced by methyltransferase like 3 (METTL3) with Graves' disease (GD).

Methods

Differently expressed genes (DEG) in GD tissues were identified using microarray analysis and further validated using CD4⁺ T cell microarray of GD tissues and isolated peripheral blood mononuclear cells (PBMCs). Furthermore, expressions of METTL3 targeted genes were detected using METTL3 knock-down experiment in RAW264.7 cells.

Results

High throughput microarrays revealed that METTL3 and

SUBMITTER: Song RH 

PROVIDER: S-EPMC8488398 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature