Transdermal vaccination via 3D-printed microneedles induces potent humoral and cellular immunity.

ABSTRACT: Vaccination is an essential public health measure for infectious disease prevention. The exposure of the immune system to vaccine formulations with the appropriate kinetics is critical for inducing protective immunity. In this work, faceted microneedle arrays were designed and fabricated utilizing a three-dimensional (3D)-printing technique called continuous liquid interface production (CLIP). The faceted microneedle design resulted in increased surface area as compared with the smooth square pyramidal design, ultimately leading to enhanced surface coating of model vaccine components (ovalbumin and CpG). Utilizing fluorescent tags and live-animal imaging, we evaluated in vivo cargo retention and bioavailability in mice as a function of route of delivery. Compared with subcutaneous bolus in

SUBMITTER: Caudill C 

PROVIDER: S-EPMC8488660 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature