Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase.

ABSTRACT: Recent advances in serial femtosecond X-ray crystallography (SFX) using X-ray free-electron lasers have paved the way for determining radiation-damage-free protein structures under nonfreezing conditions. However, the large-scale preparation of high-quality microcrystals of uniform size is a prerequisite for SFX, and this has been a barrier to its widespread application. Here, a convenient method for preparing high-quality microcrystals of a bacterial quinoprotein enzyme, copper amine oxidase from Arthrobacter globiformis, is reported. The method consists of the mechanical crushing of large crystals (5-15 mm³), seeding the crushed crystals into the enzyme solution and standing for 1 h at an ambient temperature of ∼26°C, leading to the rapid formation of microcrystals with a unif

SUBMITTER: Murakawa T 

PROVIDER: S-EPMC8488853 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature