Crystal structure determination of the armadillo repeat domain of Drosophila SARM1 using MIRAS phasing.

ABSTRACT: The crystal structure determination of the armadillo repeat motif (ARM) domain of Drosophila SARM1 (dSARM1^ARM) is described, which required the combination of a number of sources of phase information in order to obtain interpretable electron-density maps. SARM1 is a central executioner of programmed axon degeneration, a common feature of the early phase of many neurodegenerative diseases. SARM1 is held in the inactive state in healthy axons by its N-terminal auto-inhibitory ARM domain, and is activated to cleave NAD upon injury, triggering subsequent axon degeneration. To characterize the molecular mechanism of SARM1 activation, it was sought to determine the crystal structure of the SARM1 ARM domain. Here, the recombinant production and crystallization of dSARM1^ARM i

SUBMITTER: Gu W 

PROVIDER: S-EPMC8488856 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature