Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography.

ABSTRACT: Metalloproteins catalyze a range of reactions, with enhanced chemical functionality due to their metal cofactor. The reaction mechanisms of metalloproteins have been experimentally characterized by spectroscopy, macromolecular crystallography and cryo-electron microscopy. An important caveat in structural studies of metalloproteins remains the artefacts that can be introduced by radiation damage. Photoreduction, radiolysis and ionization deriving from the electromagnetic beam used to probe the structure complicate structural and mechanistic interpretation. Neutron protein diffraction remains the only structural probe that leaves protein samples devoid of radiation damage, even when data are collected at room temperature. Additionally, neutron protein crystallography provides information on

SUBMITTER: Schroder GC 

PROVIDER: S-EPMC8489226 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature