Cryo-EM single-particle structure refinement and map calculation using Servalcat.

ABSTRACT: In 2020, cryo-EM single-particle analysis achieved true atomic resolution thanks to technological developments in hardware and software. The number of high-resolution reconstructions continues to grow, increasing the importance of the accurate determination of atomic coordinates. Here, a new Python package and program called Servalcat is presented that is designed to facilitate atomic model refinement. Servalcat implements a refinement pipeline using the program REFMAC5 from the CCP4 package. After the refinement, Servalcat calculates a weighted F_o - F_c difference map, which is derived from Bayesian statistics. This map helps manual and automatic model building in real space, as is common practice in crystallography. The F_o - F_c map helps in the

SUBMITTER: Yamashita K 

PROVIDER: S-EPMC8489229 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature