Pilot-scale production of expansile nanoparticles: Practical methods for clinical scale-up.

ABSTRACT: One of the foremost challenges in translating nanoparticle technologies to the clinic is the requirement to produce materials on a large-scale. Scaling nanoparticle production methods is often non-trivial, and the success of these endeavors is frequently governed by whether or not an intermediate level of production, i.e., "pilot-scale" production, can be achieved. Pilot-scale production at the one-liter scale serves as a proof-of-concept that large-scale production will be possible. Here, we describe the pilot-scale production of the expansile nanoparticle (eNP) technology including verification of activity and efficacy following scaleup. We describe the challenges of sonication-based emulsification procedures and how these were overcome by use of a Microfluidizer technology. We also desc

SUBMITTER: Colby AH 

PROVIDER: S-EPMC8489532 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature