Enhancing the multi-attribute method through an automated and high-throughput sample preparation.

ABSTRACT: The multi-attribute method (MAM), a recent advance in the application of liquid chromatography-mass spectrometry within the pharmaceutical industry, enables the simultaneous monitoring of multiple product quality attributes in a single analytical method. While MAM is coupled with automated data processing and reporting, the sample preparation, based on proteolytic peptide mapping, remains cumbersome and low throughput. The standard sample preparation for MAM relies on protein denaturation, reduction, and alkylation prior to proteolytic digestion, but often a desalting step is required to maintain enzymatic activity. While most of the sample preparation can be automated on a standard robotic liquid handling system, a streamlined approach for protein desalting and temperature modulation is r

SUBMITTER: Sitasuwan P 

PROVIDER: S-EPMC8489909 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature