Neuromedin B receptor stimulation of Cav3.2 T-type Ca²⁺ channels in primary sensory neurons mediates peripheral pain hypersensitivity.

ABSTRACT: Background: Neuromedin B (Nmb) is implicated in the regulation of nociception of sensory neurons. However, the underlying cellular and molecular mechanisms remain unknown. Methods: Using patch clamp recording, western blot analysis, immunofluorescent labelling, enzyme-linked immunosorbent assays, adenovirus-mediated shRNA knockdown and animal behaviour tests, we studied the effects of Nmb on the sensory neuronal excitability and peripheral pain sensitivity mediated by Cav3.2 T-type channels. Results: Nmb reversibly and concentration-dependently increased T-type channel currents (I_T) in small-sized trigeminal ganglion (TG) neurons through the activation of neuromedin B receptor (NmbR). This NmbR-mediated I_T response was G_q

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8490515 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature