The kinase PDK1 regulates regulatory T cell survival via controlling redox homeostasis.

ABSTRACT: Rationale: Regulatory T cells (Treg cells) play an important role in maintaining peripheral tolerance by suppressing over-activation of effector T cells. The kinase PDK1 plays a pivotal role in conventional T cell development. However, whether PDK1 signaling affects the homeostasis and function of Treg cells remains elusive. Methods: In order to evaluate the role of PDK1 in Treg cells from a genetic perspective, mice carrying the floxed PDK1 allele were crossbred with Foxp3^Cre mice to efficiently deleted PDK1 in Foxp3⁺ Treg cells. Flow cytometry was used to detect the immune cell homeostasis of WT and PDK1^fl/flFoxp3^Cre mice. RNA-seq was used to assess the differences in transcriptional expression profile

SUBMITTER: Feng P 

PROVIDER: S-EPMC8490516 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature