Exploring the Potential of Corynebacterium glutamicum to Produce the Compatible Solute Mannosylglycerate.

ABSTRACT: The compatible solute mannosylglycerate (MG) has exceptional properties in terms of protein stabilization and protection under salt, heat, and freeze-drying stresses as well as against protein aggregation. Due to these characteristics, MG possesses large potential for clinical and biotechnological applications. To achieve efficient MG production, Corynebacterium glutamicum was equipped with a bifunctional MG synthase (encoded by mgsD and catalyzing the condensation of 3-phosphoglycerate and GDP-mannose to MG) from Dehalococcoides mccartyi. The resulting strain C. glutamicum (pEKEx3 mgsD) intracellularly accumulated about 111 mM MG (60 ± 9 mg g_CDW ^-1) with 2% glucose as a carbon source. To enable efficient mannose metabolization, the

SUBMITTER: Schwentner A 

PROVIDER: S-EPMC8490865 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature