Strategies for Rapid Identification of Acinetobacter baumannii Membrane Proteins and Polymyxin B's Effects.

ABSTRACT: Acinetobacter baumannii, especially multidrug resistant Acinetobacter baumannii, is a notable source of pressure in the areas of public health and antibiotic development. To overcome this problem, attention has been focused on membrane proteins. Different digestion methods and extraction detergents were examined for membrane proteome sample preparation, and label-free quantitative and targeted proteome analyses of the polymyxin B-induced Acinetobacter baumannii ATCC 19606 membrane proteome were performed based on nano LC-MS/MS. Ultracentrifugation of proteins at a speed of 150,000×g, digestion by trypsin, filter-aided sample preparation, and detergents such as lauryldimethylamine-N-oxide were proved as a fast and effective way for identification of membrane prot

SUBMITTER: Lu Y 

PROVIDER: S-EPMC8490878 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature