Design of Multidrug-Resistant Tuberculosis Treatment Regimens Based on DNA Sequencing.

ABSTRACT:

Background

Comprehensive and reliable drug susceptibility testing (DST) is urgently needed to provide adequate treatment regimens for patients with multidrug-resistant/rifampicin-resistant tuberculosis (MDR/RR-TB). We determined whether next-generation sequencing (NGS) analysis of Mycobacterium tuberculosis complex isolates and genes implicated in drug resistance can guide the design of effective MDR/RR-TB treatment regimens.

Methods

NGS-based genomic DST predictions of M. tuberculosis complex isolates from MDR/RR-TB patients admitted to a TB reference center in Germany between 1 January 2015 and 30 April 2019 were compared with phenotypic DST results of mycobacteria growth indicator tubes (MGIT). Standardized treatment algorithms were applied to design individualized therap

SUBMITTER: Grobbel HP 

PROVIDER: S-EPMC8492214 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature