Nestin Is Required for Spindle Assembly and Cell-Cycle Progression in Glioblastoma Cells.

ABSTRACT: Nestin, a class IV intermediate filament protein, is generally considered as a putative marker of neural stem and progenitor cells in the central nervous system. Glioma is a common type of adult brain tumors, and glioblastoma (GBM) represents the most aggressive form of glioma. Here, we report that Nestin expression is significantly upregulated in human GBM, compared with other types of glioma. Nestin knockdown or deletion in U251 cells and tumor cells from GBM patients derived xenografts resulted in G₂-M arrest, finally leading to apoptosis in tumor cells. Using proximity-dependent biotin identification method, we identified βII-tubulin as an interacting protein of Nestin in U251 cells. Nestin stabilized βII-tubulin in U251 cells through physical interaction. Knockdown of Nesti

SUBMITTER: Wang Q 

PROVIDER: S-EPMC8492506 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature