Elevated N-Linked Glycosylation of IgG V Regions in Myasthenia Gravis Disease Subtypes.

ABSTRACT: Elevated N-linked glycosylation of IgG V regions (IgG-V^N-Glyc) is an emerging molecular phenotype associated with autoimmune disorders. To test the broader specificity of elevated IgG-V^N-Glyc, we studied patients with distinct subtypes of myasthenia gravis (MG), a B cell-mediated autoimmune disease. Our experimental design focused on examining the B cell repertoire and total IgG. It specifically included adaptive immune receptor repertoire sequencing to quantify and characterize N-linked glycosylation sites in the circulating BCR repertoire, proteomics to examine glycosylation patterns of the total circulating IgG, and an exploration of human-derived recombinant autoantibodies, which were studied with mass spectrometry and Ag binding assays to respectively confirm occ

SUBMITTER: Mandel-Brehm C 

PROVIDER: S-EPMC8492536 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature