Evolution of Cytomegalovirus-Responsive T Cell Clonality following Solid Organ Transplantation.

ABSTRACT: CMV infection is a significant complication after solid organ transplantation. We used single cell TCR αβ sequencing to determine how memory inflation impacts clonality and diversity of the CMV-responsive CD8 and CD4 T cell repertoire in the first year after transplantation in human subjects. We observed CD8 T cell inflation but no changes in clonal diversity, indicating homeostatic stability in clones. In contrast, the CD4 repertoire was diverse and stable over time, with no evidence of CMV-responsive CD4 T cell expansion. We identified shared CDR3 TCR motifs among patients but no public CMV-specific TCRs. Temporal changes in clonality in response to transplantation and in the absence of detectable viral reactivation suggest changes in the repertoire immediately after transplantation foll

SUBMITTER: Higdon LE 

PROVIDER: S-EPMC8492537 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature