Synthesis of [1-¹³ C-5-¹² C]-alpha-ketoglutarate enables noninvasive detection of 2-hydroxyglutarate.

ABSTRACT: Isocitrate dehydrogenase 1 (IDH1) mutations that generate the oncometabolite 2-hydroxyglutarate (2-HG) from α-ketoglutarate (α-KG) have been identified in many types of tumors and are an important prognostic factor in gliomas. 2-HG production can be determined by hyperpolarized carbon-13 magnetic resonance spectroscopy (HP-¹³ C-MRS) using [1-¹³ C]-α-KG as a probe, but peak contamination from naturally occurring [5-¹³ C]-α-KG overlaps with the [1-¹³ C]-2-HG peak. Via a newly developed oxidative-Stetter reaction, [1-¹³ C-5-¹² C]-α-KG was synthesized. α-KG metabolism was measured via HP-¹³ C-MRS using [1-¹³ C-5-¹² C]-α-KG as a probe. [1-¹³ C-5-¹² C]-α-KG was synthesized in

SUBMITTER: Miura N 

PROVIDER: S-EPMC8492538 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature