Lung microbiome of stable and exacerbated COPD patients in Tshwane, South Africa.

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is characterised by the occurrence of exacerbations triggered by infections. The aim of this study was to determine the composition of the lung microbiome and lung virome in patients with COPD in an African setting and to compare their composition between the stable and exacerbated states. Twenty-four adult COPD patients were recruited from three hospitals. Sputum was collected and bacterial DNA was extracted. Targeted metagenomics was performed to determine the microbiome composition. Viral DNA and RNA were extracted from selected samples followed by cDNA conversion. Shotgun metagenomics sequencing was performed on pooled DNA and RNA. The most abundant phyla across all samples were Firmicutes and Proteobacteria. The following genera were most p

SUBMITTER: Goolam Mahomed T 

PROVIDER: S-EPMC8492659 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature