Evaluation of a genus-specific rGroEL_1-524 IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand.

ABSTRACT: In the present study, we developed a genus-specific rGroEL_1-524 IgM-ELISA assay for use in screening diagnosis of suspected leptospirosis among acute undifferentiated febrile illness patients during acute fever. The diagnostic accuracies of the rGroEL_1-524 IgM-ELISA, commercial Panbio IgM-ELISA, and Virion-Serion Classic IgG-ELISA were evaluated using 133 Thai leptospirosis sera and 210 controls. Sensitivities were 91.7%, 59.6%, and 17.7% for acute infection, and the specificities were 92.6%, 90.2%, and 88.3% for the non-leptospirosis control, respectively. The rGroEL_1-524 IgM-ELISA had high sensitivity, at 92.3% and 91.7%, among culture-positive and MAT-negative cases at 1-3 days post-onset of symptoms (DPO1-3), respectively. Impaired specificity on scrub

SUBMITTER: Maneewatchararangsri S 

PROVIDER: S-EPMC8492722 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature