Age and sex modify cellular proliferation responses to oxidative stress and glucocorticoid challenges in baboon cells.

ABSTRACT: Aging is associated with progressive loss of cellular homeostasis resulting from intrinsic and extrinsic challenges. Lack of a carefully designed, well-characterized, precise, translational experimental model is a major limitation to understanding the cellular perturbations that characterize aging. Here, we tested the feasibility of primary fibroblasts isolated from nonhuman primates (baboons) as a model of cellular resilience in response to homeostatic challenge. Using a real-time live-cell imaging system, we precisely defined a protocol for testing effects of prooxidant compounds (e.g., hydrogen peroxide (H₂O₂), paraquat), thapsigargin, dexamethasone, and a low glucose environment on cell proliferation in fibroblasts derived from baboons across the life course (n = 

SUBMITTER: Adekunbi DA 

PROVIDER: S-EPMC8492861 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature