Synthesis of bioluminescent gold nanoparticle-luciferase hybrid systems for technological applications.

ABSTRACT: Bioluminescent gold nanoparticles (AuNPs) were synthesized in situ using dithiol-terminated polyethylene glycol (PEG(SH)₂) as reducer and stabilizing agents. Hybrid Au/F₃O₄ nanoparticles were also produced in a variation of synthesis, and both types of nanostructures had the polymer capping replaced by L-cysteine (Cys). The four types of nanoparticles, PEG(SH)₂AuNPs, PEG(SH)₂Au/F₃O₄NPs, CysAuNPs, and CysAu/F₃O₄NPs were associated with purified recombinant Pyrearinus termitilluminans green emitting click beetle luciferase (PyLuc) and Phrixotrix hirtus (RELuc) red-emitting railroad worm luciferase. Enzyme association with PEG(SH)₂ was also investigated as a control. Luciferases were

SUBMITTER: Belleti E 

PROVIDER: S-EPMC8493054 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature