Bone marrow CD73⁺ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo.

ABSTRACT: Mesenchymal stem cells (MSCs) are multipotent and considered to be of great potential for regenerative medicine. We could show recently (Breitbach, Kimura et al. 2018) that a subpopulation of MSCs as well as sinusoidal endothelial cells (sECs) in the bone marrow (BM) of CD73-EGFP reporter mice could be labeled in vivo. We took advantage of this model to explore the plasticity and osteogenic potential of CD73-EGFP⁺ MSCs in vitro and their role in the regenerative response upon bone lesion in vivo. Herein we show that isolated CD73-EGFP⁺ MSCs displayed more pronounced stemness and stronger in vitro differentiation capacity into the osteogenic lineage compared to CD73-EGFP^- MSCs. In a bone fracture model, endogenous BM-resident CD73

SUBMITTER: Kimura K 

PROVIDER: S-EPMC8493579 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature