Synchrony of sarcomeric movement regulates left ventricular pump function in the in vivo beating mouse heart.

ABSTRACT: Sarcomeric contraction in cardiomyocytes serves as the basis for the heart's pump functions. It has generally been considered that in cardiac muscle as well as in skeletal muscle, sarcomeres equally contribute to myofibrillar dynamics in myocytes at varying loads by producing similar levels of active and passive force. In the present study, we expressed α-actinin-AcGFP in Z-disks to analyze dynamic behaviors of sequentially connected individual sarcomeres along a myofibril in a left ventricular (LV) myocyte of the in vivo beating mouse heart. To quantify the magnitude of the contribution of individual sarcomeres to myofibrillar dynamics, we introduced the novel parameter "contribution index" (CI) to measure the synchrony in movements between a sarcomere and a myofibril (from -1 [complete a

SUBMITTER: Kobirumaki-Shimozawa F 

PROVIDER: S-EPMC8493835 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature