AOAC-OMA/MicroVal Harmonized Validation of Peel PlateTM EB (Enterobacteriaceae Bacteria), First Action 2018.05.

ABSTRACT:

Background

Peel PlateTM  Enterobacteriaceae Bacteria (EB) is dried selective media on a 47 mm plastic plate that produces enzyme substrate colored colonies on rehydration and incubation for 24 h and up to 48 h at 37 ± 1°C.

Purpose

The method validation compared quantification of EB to reference methods ISO 21528:2017 Parts 1 and 2.

Methods

Matrixes compared were whole milk, skim powdered milk, vanilla ice cream, butter, infant formulas (soy- and dairy-based), infant cereals ± probiotic, environmental sponge swab of stainless steel surface, and poultry carcass rinse with two different peptone buffers.

Results

In inclusivity and exclusivity studies, the method detected 54 of 54 EB strains and did not detect 30 of 30 non-EB strains. In matrix studies, the claimed

SUBMITTER: Salter RS 

PROVIDER: S-EPMC8493887 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature