Compartmentation of β₂ -adrenoceptor stimulated cAMP responses by phosphodiesterase types 2 and 3 in cardiac ventricular myocytes.

ABSTRACT:

Background and purpose

In cardiac myocytes, cyclic AMP (cAMP) produced by both β₁ - and β₂ -adrenoceptors increases L-type Ca²⁺ channel activity and myocyte contraction. However, only cAMP produced by β₁ -adrenoceptors enhances myocyte relaxation through phospholamban-dependent regulation of the sarco/endoplasmic reticulum Ca²⁺ ATPase 2 (SERCA2). Here we have tested the hypothesis that stimulation of β₂ -adrenoceptors produces a cAMP signal that is unable to reach SERCA2 and determine what role, if any, phosphodiesterase (PDE) activity plays in this compartmentation.

Experimental approach

The cAMP responses produced by β₁ -and β₂ -adrenoceptor stimulation were studied in adult rat ventric

SUBMITTER: Rudokas MW 

PROVIDER: S-EPMC8493936 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature