Broad-range RNA modification analysis of complex biological samples using rapid C18-UPLC-MS.

ABSTRACT: Post-transcriptional RNA modifications play an important role in cellular metabolism with homoeostatic disturbances manifesting as a wide repertoire of phenotypes, reduced stress tolerance and translational perturbation, developmental defects, and diseases, such as type II diabetes, leukaemia, and carcinomas. Hence, there has been an intense effort to develop various methods for investigating RNA modifications and their roles in various organisms, including sequencing-based approaches and, more frequently, liquid chromatography-mass spectrometry (LC-MS)-based methods. Although LC-MS offers numerous advantages, such as being highly sensitive and quantitative over a broad detection range, some stationary phase chemistries struggle to resolve positional isomers. Furthermore, the demand for de

SUBMITTER: Gregorova P 

PROVIDER: S-EPMC8494288 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature