Activity-dependent Golgi satellite formation in dendrites reshapes the neuronal surface glycoproteome.

ABSTRACT: Activity-driven changes in the neuronal surface glycoproteome are known to occur with synapse formation, plasticity, and related diseases, but their mechanistic basis and significance are unclear. Here, we observed that N-glycans on surface glycoproteins of dendrites shift from immature to mature forms containing sialic acid in response to increased neuronal activation. In exploring the basis of these N-glycosylation alterations, we discovered that they result from the growth and proliferation of Golgi satellites scattered throughout the dendrite. Golgi satellites that formed during neuronal excitation were in close association with endoplasmic reticulum (ER) exit sites and early endosomes and contained glycosylation machinery without the Golgi structural protein, GM130. They

SUBMITTER: Govind AP 

PROVIDER: S-EPMC8494481 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature