Chromatographic Fingerprinting Based on Column Switching Technology for Quality Evaluation of Tianmeng Oral Liquid.

ABSTRACT: Separation power was limited when the conventional high-performance liquid chromatography (HPLC) fingerprinting method based on a single column was used to analyze very complex traditional Chinese medicine (TCM) preparations. In this research, a novel HPLC fingerprinting method based on column switching technology by using a single pump was established for evaluating the quality of Tianmeng oral liquid (TMOL). Twelve batches of TMOL samples were used for constructing HPLC fingerprints. Compared with the 16 common peaks in fingerprinting with a single column, 25 common peaks were achieved with two columns connected through a six-way valve. The similarity analysis combined with bootstrap method was applied to determine the similarity threshold, which was 0.992 to distinguish expired samples

SUBMITTER: Yuan M 

PROVIDER: S-EPMC8494583 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature