Spatially resolved cell atlas of the mouse primary motor cortex by MERFISH.

ABSTRACT: A mammalian brain is composed of numerous cell types organized in an intricate manner to form functional neural circuits. Single-cell RNA sequencing allows systematic identification of cell types based on their gene expression profiles and has revealed many distinct cell populations in the brain^1,2. Single-cell epigenomic profiling^3,4 further provides information on gene-regulatory signatures of different cell types. Understanding how different cell types contribute to brain function, however, requires knowledge of their spatial organization and connectivity, which is not preserved in sequencing-based methods that involve cell dissociation. Here we used a single-cell transcriptome-imaging method, multiplexed error-robust fluorescence in situ hybridization (MERFISH)

SUBMITTER: Zhang M 

PROVIDER: S-EPMC8494645 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature