Genetic dissection of the glutamatergic neuron system in cerebral cortex.

ABSTRACT: Diverse types of glutamatergic pyramidal neurons mediate the myriad processing streams and output channels of the cerebral cortex^1,2, yet all derive from neural progenitors of the embryonic dorsal telencephalon^3,4. Here we establish genetic strategies and tools for dissecting and fate-mapping subpopulations of pyramidal neurons on the basis of their developmental and molecular programs. We leverage key transcription factors and effector genes to systematically target temporal patterning programs in progenitors and differentiation programs in postmitotic neurons. We generated over a dozen temporally inducible mouse Cre and Flp knock-in driver lines to enable the combinatorial targeting of major progenitor types and projection classes. Combinatorial strategies confer vi

SUBMITTER: Matho KS 

PROVIDER: S-EPMC8494647 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature