A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex.

ABSTRACT: Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain^1-3. With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. The resulting reference atlas-containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modali

SUBMITTER: Yao Z 

PROVIDER: S-EPMC8494649 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature