Characterisation of enterovirus RNA detected in the pancreas and other specimens of live patients with newly diagnosed type 1 diabetes in the DiViD study.

ABSTRACT:

Aims/hypothesis

The Diabetes Virus Detection (DiViD) study is the first study to laparoscopically collect pancreatic tissue and purified pancreatic islets together with duodenal mucosa, serum, peripheral blood mononuclear cells (PBMCs) and stools from six live adult patients (age 24-35 years) with newly diagnosed type 1 diabetes. The presence of enterovirus (EV) in the pancreatic islets of these patients has previously been reported.

Methods

In the present study we used reverse transcription quantitative real-time PCR (RT-qPCR) and sequencing to characterise EV genomes present in different tissues to understand the nature of infection in these individuals.

Results

All six patients were found to be EV-positive by RT-qPCR in at least one of the tested sample types. Four

SUBMITTER: Oikarinen S 

PROVIDER: S-EPMC8494699 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature