Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer.

ABSTRACT: In colorectal cancer, oncogenic mutations transform a hierarchically organized and homeostatic epithelium into invasive cancer tissue lacking visible organization. We sought to define transcriptional states of colorectal cancer cells and signals controlling their development by performing single-cell transcriptome analysis of tumors and matched non-cancerous tissues of twelve colorectal cancer patients. We defined patient-overarching colorectal cancer cell clusters characterized by differential activities of oncogenic signaling pathways such as mitogen-activated protein kinase and oncogenic traits such as replication stress. RNA metabolic labeling and assessment of RNA velocity in patient-derived organoids revealed developmental trajectories of colorectal cancer cells organized along a mit

SUBMITTER: Uhlitz F 

PROVIDER: S-EPMC8495451 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature