DNA replication, transcription, and H3K56 acetylation regulate copy number and stability at tandem repeats.

ABSTRACT: Tandem repeats are inherently unstable and exhibit extensive copy number polymorphisms. Despite mounting evidence for their adaptive potential, the mechanisms associated with regulation of the stability and copy number of tandem repeats remain largely unclear. To study copy number variation at tandem repeats, we used two well-studied repetitive arrays in the budding yeast genome, the ribosomal DNA (rDNA) locus, and the copper-inducible CUP1 gene array. We developed powerful, highly sensitive, and quantitative assays to measure repeat instability and copy number and used them in multiple high-throughput genetic screens to define pathways involved in regulating copy number variation. These screens revealed that rDNA stability and copy number are regulated by DNA replication, transcription, a

SUBMITTER: Salim D 

PROVIDER: S-EPMC8495749 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature