RNA-Seq based transcriptome analysis in oral lichen planus.

ABSTRACT:

Objectives

Oral lichen planus (OLP) is a T cell-mediated autoimmune disease recognized as an oral potential malignant disorder (OPMD) with the precise mechanism unknown. This study focused on the transcriptional profiles of OLP to elucidate its potential pathogenesis.

Methods

We conducted RNA sequencing on matched 6 OLP tissues and 6 normal oral mucosal tissues. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and weighted gene co-expression network analysis (WGCNA) were performed on differentially expressed genes (DEGs). We utilized qRT-PCR to validated the top dysregulated genes and hub genes in another 10 pairs of specimens.

Results

A total of 153 DEGs (p-values< 0.05) were detected from RNA-Seq. According to GO and KEG

SUBMITTER: Wang H 

PROVIDER: S-EPMC8495917 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature