Genome-wide analysis of the soybean root transcriptome reveals the impact of nitrate on alternative splicing.

ABSTRACT: In plants, nitrate acts not only as a signaling molecule that affects plant development but also as a nutrient. The development of plant roots, which directly absorb nutrients, is greatly affected by nitrate supply. Alternative gene splicing plays a crucial role in the plant stress response by increasing transcriptome diversity. The effects of nitrate supply on alternative splicing (AS), however, have not been investigated in soybean roots. We used high-quality high-throughput RNA-sequencing data to investigate genome-wide AS events in soybean roots in response to various levels of nitrate supply. In total, we identified 355 nitrate-responsive AS events between optimal and high nitrate levels (NH), 335 nitrate-responsive AS events between optimal and low nitrate levels (NL), and 588 nitrat

SUBMITTER: Guo B 

PROVIDER: S-EPMC8495941 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature