Computational design and experimental characterization of a photo-controlled mRNA-cap guanine-N7 methyltransferase.

ABSTRACT: The spatial and temporal control of gene expression at the post-transcriptional level is essential in eukaryotic cells and developing multicellular organisms. In recent years optochemical and optogenetic tools have enabled the manipulation and investigation of many steps in the involved processes. However, examples for light-mediated control of eukaryotic mRNA processing and the responsible enzymes are still rare. In particular, methylation of the 5' cap of mRNA is required for ribosome assembly, and the responsible guanine-N7 methyltransferase (MTase) from E. cuniculi (Ecm1) proved suitable for activating translation. Here, we report on a photoswitchable MTase obtained by bridging the substrate-binding cleft of Ecm1 with a tetra-ortho-methoxy-azobenzene. This azobenzene deri

SUBMITTER: Reichert D 

PROVIDER: S-EPMC8495969 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature