Segmental and site-specific isotope labelling strategies for structural analysis of posttranslationally modified proteins.

ABSTRACT: Posttranslational modifications can alter protein structures, functions and locations, and are important cellular regulatory and signalling mechanisms. Spectroscopic techniques such as nuclear magnetic resonance, infrared and Raman spectroscopy, as well as small-angle scattering, can provide insights into the structural and dynamic effects of protein posttranslational modifications and their impact on interactions with binding partners. However, heterogeneity of modified proteins from natural sources and spectral complexity often hinder analyses, especially for large proteins and macromolecular assemblies. Selective labelling of proteins with stable isotopes can greatly simplify spectra, as one can focus on labelled residues or segments of interest. Employing chemical biology tools for mod

SUBMITTER: Vogl DP 

PROVIDER: S-EPMC8496066 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature