Transgenesis of mammalian PABP reveals mRNA polyadenylation as a general stress response mechanism in bacteria.

ABSTRACT: In eukaryotes, mRNA 3'-polyadenylation triggers poly(A) binding protein (PABP) recruitment and stabilization. In a stark contrast, polyadenylation marks mRNAs for degradation in bacteria. To study this difference, we trans-express the mammalian nuclear PABPN1 chromosomally and extra-chromosomally in Escherichia coli. Expression of PABPN1 but not the mutant PABPN1 stabilizes polyadenylated mRNAs and improves their half-lives. In the presence of PABPN1, 3'-exonuclease PNPase is not detected on PA-tailed mRNAs compromising the degradation. We show that PABPN1 trans-expression phenocopies pcnB (that encodes poly(A) polymerase, PAPI) mutation and regulates plasmid copy number. Genome-wide RNA-seq analysis shows a general up-regulation of polyadenylated mRNAs on PABPN1 expression,

SUBMITTER: Francis N 

PROVIDER: S-EPMC8496165 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature