Charting oncogenicity of genes and variants across lineages via multiplexed screens in teratomas.

ABSTRACT: Deconstructing tissue-specific effects of genes and variants on proliferation is critical to understanding cellular transformation and systematically selecting cancer therapeutics. This requires scalable methods for multiplexed genetic screens tracking fitness across time, across lineages, and in a suitable niche, since physiological cues influence functional differences. Towards this, we present an approach, coupling single-cell cancer driver screens in teratomas with hit enrichment by serial teratoma reinjection, to simultaneously screen drivers across multiple lineages in vivo. Using this system, we analyzed population shifts and lineage-specific enrichment for 51 cancer associated genes and variants, profiling over 100,000 cells spanning over 20 lineages, across two rounds of serial re

SUBMITTER: Parekh U 

PROVIDER: S-EPMC8496177 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature