Engineering threshold-based selection systems.

ABSTRACT: Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.

SUBMITTER: Pedone KH 

PROVIDER: S-EPMC8496214 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature