Efficient construction of a linkage map and haplotypes for Mentha suaveolens using sequence capture.

ABSTRACT: The sustainability of many crops is hindered by the lack of genomic resources and a poor understanding of natural genetic diversity. Particularly, application of modern breeding requires high-density linkage maps that are integrated into a highly contiguous reference genome. Here, we present a rapid method for deriving haplotypes and developing linkage maps, and its application to Mentha suaveolens, one of the diploid progenitors of cultivated mints. Using sequence-capture via DNA hybridization to target single nucleotide polymorphisms (SNPs), we successfully genotyped ∼5000 SNPs within the genome of >400 individuals derived from a self cross. After stringent quality control, and identification of nonredundant SNPs, 1919 informative SNPs were retained for linkage map construction. The resu

SUBMITTER: Tsai H 

PROVIDER: S-EPMC8496254 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature