Methylotroph Quorum Sensing Signal Identification by Inverse Stable Isotopic Labeling.

ABSTRACT: Natural products are an essential source of bioactive compounds. Isotopic labeling is an effective way to identify natural products that incorporate a specific precursor; however, this approach is limited by the availability of isotopically enriched precursors. We used an inverse stable isotopic labeling approach to identify natural products by growing bacteria on a ¹³C-carbon source and then identifying ¹²C-precursor incorporation by mass spectrometry. We applied this approach to methylotrophs, ecologically important bacteria predicted to have significant yet underexplored biosynthetic potential. We demonstrate that this method identifies N-acyl homoserine lactone quorum sensing signals produced by diverse methylotrophs grown on three different one-carbon comp

SUBMITTER: Cummings DA 

PROVIDER: S-EPMC8496459 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature