Quantification of natural abundance NMR data differentiates the solution behavior of monoclonal antibodies and their fragments.

ABSTRACT: Biotherapeutics are an important class of molecules for the treatment of a wide range of diseases. They include low molecular weight peptides, highly engineered protein scaffolds and monoclonal antibodies. During their discovery and development, assessments of the biophysical attributes is critical to understanding the solution behavior of therapeutic proteins and for de-risking liabilities. Thus, methods that can quantify, characterize, and provide a basis to inform risks and drive the selection of more optimal antibody and alternative scaffolds are needed. Nuclear Magnetic Resonance (NMR) spectroscopy is a technique that provides a means to probe antibody and antibody-like molecules in solution, at atomic resolution, under any formulated conditions. Here, all samples were profiled at nat

SUBMITTER: Ban D 

PROVIDER: S-EPMC8496538 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature