Accumulation of Abnormal Amyloplasts in Pulp Cells Induces Bitter Pit in Malus domestica.

ABSTRACT: Apple bitter pit primarily occurs during fruit ripening and storage; however, its formation mechanism remains unclear. Although it is considered that Ca²⁺ deficiency causes metabolic disorders in apples, there have been few studies on the mechanism of the bitter pit from the perspective of cell structure. At the fruit ripening stage, the fruit with a bitter pit on the tree was taken as the research material. In this study, the microscopic observation revealed numerous amyloplasts in the pulp cells of apples affected with bitter pit, but not in the healthy pulp. Furthermore, the results of fluorescence staining and transmission electron microscopy (TEM) revealed that the bitter pit pulp cells undergo programmed cell death (PCD), their nuclear chromosomes condense, and amyloplast

SUBMITTER: Qiu L 

PROVIDER: S-EPMC8496688 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature