Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells.

ABSTRACT: The ability to study human cardiac development in health and disease is highly limited by the capacity to model the complexity of the human heart in vitro. Developing more efficient organ-like platforms that can model complex in vivo phenotypes, such as organoids and organs-on-a-chip, will enhance the ability to study human heart development and disease. This paper describes a protocol to generate highly complex human heart organoids (hHOs) by self-organization using human pluripotent stem cells and stepwise developmental pathway activation using small molecule inhibitors. Embryoid bodies (EBs) are generated in a 96-well plate with round-bottom, ultra-low attachment wells, facilitating suspension culture of individualized constructs. The EBs undergo differentiation into hHOs by a three-ste

SUBMITTER: Lewis-Israeli YR 

PROVIDER: S-EPMC8496916 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature