Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.

ABSTRACT: Serum-derived extracellular vesicles (EVs) are a promising source of biomarkers; however, major challenges in EV separation and proteomic profiling remain for isolating EVs from a small amount, that is, on the microliter scale, of human serum while minimizing the contamination of blood proteins and lipoprotein particles coeluting in EV preparations. Herein we have developed a column-based CD9-antibody-immobilized high-performance liquid chromatography immunoaffinity chromatography(CD9-HPLC-IAC) technology for EV isolation from a microliter scale of serum for downstream proteomic analysis. The CD9-HPLC-IAC method achieved EV isolation from 40 μL of serum in 30 min with a yield of 8.0 × 10⁹ EVs, where EVs were further processed with a postcolumn cleaning step using the 50 kDa mole

SUBMITTER: Zhu J 

PROVIDER: S-EPMC8496948 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature